Progress in generating, controlling and detecting photons continues to spawn new theories, technologies and innovative designs of computer-controlled light microscopes with improved spatio-temporal resolution and sensitivity.
I will present advanced microscopy schemes based on steering of laser beams with dynamic diffractive optical elements. In combination with engineered molecules, such microscopes have become powerful tools for structural and functional analysis of biological tissue including the living brain. The complexity of brain tissue requires scalable approaches which I will briefly discuss.