Type of presentation: Poster

MS-7-P-2118 Fibrous scaffolds of PLA and PLA/HA for bone tissue

Vargas-Becerril N.1, Granados-Hernández M. V.1, Téllez-Jurado L.2, Hipólito-García M.3, Álvarez-Gregoso O.3, Álvarez-Pérez M. A.1
1Laboratorio de Bioingeniería de Tejidos; División de Estudios de Posgrado e Investigación de la Facultad de Odontología, UNAM. Circuito Exterior s/n. Cd. Universitaria, 04510 Coyoacán México D. F., México., 22Departamento de Ingeniería Metalúrgica y de Materiales E.S.I.Q.I.E-I.P.N, Unidad Professional Adolfo López Mateos 7, Lindavista. 07738 Gustavo A. Madero, México D. F., México. , 3Instituto de Investigaciones en Materiales, Circuito Exterior s/n. Cd. Universitaria, 04510 Coyoacán México D. F., México.
nancyvb09@gmail.com

Scalffolds have been became an important materials for the tissue engineering, which provide temporal mechanical and structural support to cells [1]. The scalffolding materials for bone tissue should be osteoconductive such that osteoprogenitor cells can adhere and migrate on the scalffolds, differentiate, and finally form new bone. Some inorganic materials have been incorporated to polymeric scalffolds improving, the properties like bioactivity and biological functions [2].
In the present work we present poly (lactic acid) (PLA) and poly (lactic acid)/hydroxyapatite (PLA/HA) hybrid scalffolds. They were prepared by jet spinning, using five concentrations of PLA: 2.25, 6.25, 7.25 and 10 %, and 1g of HA was added to form the hybrid scalffolds. Morphology and surface were characterized by scanning electron microscopy (SEM), Atomic force microscopy (AFM). Thermal properties were carried out by Thermo gravimetric analysis (TGA) and infrared spectroscopy (FT-IR) was employed to analyze the atomic structure.
SEM images (figure1), show the effect of HA on the structure of PLA scalffolds. The width of PLA/HA scalffolds increase. The morphology changes from scalffolds like fibers to fibers with a form like staggered flakes. AFM images displayed higher roughness on the surface of hybrid scalffolds. FTIR of the hybrid scalffolds display the bands attributed to PO4+3 groups, suggesting the incorporation of HA into the organic PLA matrix. Thermal properties change with the incorporation of HA into the matrix of PLA.

References

[1] Nora Nseir, Omri Regev et al, Tissue Engineering: Part C, Volume 19, 4, (2013).

[2] Abdalla Abdal-hay et al, Colloids and Surfaces B:Biointerfases 102 (2013).

Authors want to thank to UNAM-DGAPA for postdoctoral scholarship supporting to NVB during the course of this study. This research were financially supported by funds from the UNAM-DGAPA: PAPIIT project IN213912 and Graduate Program of High Quality UNAM-CONACYT No. I010/480/2013 C-736/213.

Fig. 1: SEM images show the morphological changes when the HA is added on the PLA and hybrid schalffolds are formed by jet spinning; (a) PLA scalffolds and (b) PLA/HA hybrid scalffolds.